Autologous Cultured Chondrocytes Impregnated in ree-dimensional Biodegradable Scaffold for Chondral Defects in Rabbits – An Experimental Study

Vol 33 | Issue 3 | Sep – Dec 2018 | page: 29-34 |Shivam Sinha, Abhimanyu Madhual, Amit Rastogi, Pradeep Kumar Srivastava, Sarada Prasanna Mallick.


Authors: Shivam Sinha [1], Abhimanyu Madhual [1], Amit Rastogi [1], Pradeep Kumar Srivastava [1,2], Sarada Prasanna Mallick  [2,3]

[1] Department of Orthopaedics , Institute of Medical Sciences, Banaras Hindu University,Varanasi-221005.

[2] School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, India-221005.

[3] Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, India 522502..

Address of Correspondence
Dr. Shivam Sinha,

Assistant Professor, Dept. of Orthopaedics,

Institute of Medical Sciences,

Banaras Hindu University,Varanasi- 221005

Email: shivamortho81@gmail.com


Abstact

Background: Osteochondral lesions in young adults, if left untreated invariably progresses to degenerative joint disorders. e problem adds a burden on finances and average productivity of the patients and countries’ economics. Among the known strategies for treatment, none has proven to be gold standard, and each one is having their own pros and cons. Osteochondral tissue engineering (OTE) offers a promising future where cultured chondrocytes can be grown on biocompatible biomaterial scaffolds and implanted on defects.

Materials and Methods: We tested a novel three-dimensional scaffold using chitosan and poly-L-lactide (PLLA) in recommended proportion on animal experimental model. It was seeded with autologous chondrocytes harvested from tibial condyle of rabbits. 10 rabbits were implanted with this cell-seeded scaffold and were followed at 4, 8, and 12 weeks before being sacrificed. Histological and gross examination was graded on a rank scale. It was compared between the implanted knee and contralateral control void. Values were examined statistically.

Results: Outcome shows good quality of cartilage tissue, less fibrotic growth, beer amalgamation with the surrounding tissue, uptake at the implanted bed, and regular proliferative growth as early as 8 weeks. e scaffold imparts structural stability to the chondrocytes. The scaffold does not interfere with the normal healing process and was less inflammatory by 12 weeks.

Conclusion: OTE with bioscaffold using PLLA-chitosan and cultured chondrocytes has positive and promising outcomes in healing of cartilage defects.

Keywords: Chondrocytes, Chitosan, Poly-l-lactide, Bioscaffold, Chondral lesion, Osteochondral tissue engineering.


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How to Cite this Article: Sinha S, Madhual A, Rastogi A, Srivastava P K, Mallick S P. Autologous Cultured Chondrocytes Impregnated in ree-dimensional Biodegradable Scaffold for Chondral Defects in Rabbits – An Experimental Study. Journal of Bone and Joint Diseases Sep – Dec 2018;33(3):29-34.


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